![smada chapter 6 smada chapter 6](https://www.mdpi.com/ijms/ijms-19-02532/article_deploy/html/images/ijms-19-02532-g001-550.jpg)
These miRNAs are moderately expressed as compared to miR-16 in which saturated expression across samples prevents miR-16 from functioning as a reliable biomarker. miRNAs such as miR_17_5p, miR_191_5p, and those on the left of the Heatmap that cluster with let_7b_5p, were identified as significantly changing in expression after intervention. Relatively few miRNAs varied in expression between baseline and intervention, as was expected. Blue indicates lower signal intensity, whereas red indicates higher signal intensity. Data are expressed in a log10 scale post geNorm analysis. Heatmap depicting an equal distribution of miRNA expression (columns) that passed the detection threshold cutoff across all samples (rows).
![smada chapter 6 smada chapter 6](https://0.academia-photos.com/attachment_thumbnails/1763958/mini_magick20180818-9796-17gx5pc.png)
Unsupervised clustering of the 35 miRNAs assessed from serum samples from those enrolled in LEAN at baseline and 6 months post intervention. Heatmap denoting background-normalized expression of all miRNA probes assessed in LEAN. These data are supported by previous studies associating miRNA with obesity, and this first past analysis indicated our methodology of assessing miRNA levels from crude serum preparations was a valid measure for identifying BMI-associated miRNAs.įigure S3. Hierarchical clustering indicated certain miRNAs such as miR-133a/b were highly expressed in those patients with low BMIs, while those considered obese harbored high expression of circulating miR-122. Samples were binned into those with a BMI greater than (Green group) or less than (Red group) 30kg/m 2. Cluster analysis was performed on samples from HOPE to assess miRNA associations with BMI. Cluster analysis denoting miRNA-expression signatures in HOPE patients stratified by BMI. Those miRNAs in blue were considered lowly abundant and below the threshold of detection, and thus removed from future analysis in the LEAN study.įigure S2. An equal distribution of miRNA expression was observed across all samples (log10 scale), and indicate a number of miRNAs were robustly detectable at baseline.
![smada chapter 6 smada chapter 6](https://ars.els-cdn.com/content/image/1-s2.0-S1569190X19301649-gr9.jpg)
Unsupervised cluster analysis shows probe abundance for each miRNA in relation to the individual samples within the study. miRNA expression was background normalized via geNorm, an expression average of the most invariant miRNAs within the dataset. Heatmap depicting expression of 68 miRNAs from baseline serum samples from those enrolled in HOPE. Heatmap denoting background-normalized expression of all miRNA probes assessed in HOPE. Top canonical pathways for BMI-correlated (HOPE) & weight loss-responsive (LEAN) miRNAsįigure S1. Top ten targets of LEAN-Intervention regulated miRNAs Abbreviations: LEAN: Lifestyle, Exercise and Nutrition, BMI: body mass index, TS: TargetScan TCS: Total Context Score Top ten targets of BMI-associated miRNAs from HOPE Abbreviations: HOPE: Hormones and Physical Exercise, BMI: body mass index, TS: TargetScan TCS: Total Context Score